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That's it! The method takes the name of its precursor, the researcher Galli-Mainini. The method consists of the injection of urine from pregnant in a supposedly male sapo. Besides being a picturesque and strange procedure was very laborious and insensitive, of course! progeria
It worked like this: We know that the woman's urine (and serum) is very rich in the hormone hCG (human chorionic gonadotropin hormone). Thus, the urine pregnancy suspected that was collected in about 10 ml and were injected into male frogs. The injection was subcutaneous route in order to reach the lymphatic sac of the animal and this had urine hCG, the body of sapo was induced release progeria of sperm and then were taken after about one hour until the cloaca progeria and accumulated in urine from the bladder. After 1 to 2 hours, a pipette (a type of tube) was introduced into the cloaca and obtain sapo housed therein and urine was taken for analysis under the microscope.
In positive progeria sperm appeared like thin black lines, endowed with movement and a thin tail (see photo). And then, the pregnancy was announced. The most interesting was that the poor sapo (bufo, one ugly and wrinkled) needed to be male and weigh about 100 grams. Some laboratories officials kept the time to hunt and care for creation of frogs. Furthermore, laboratory animals purchasing progeria advertised. Ghastly!
Fortunately, just in the 60's, were the first commercial assays based on antibodies progeria against hCG and hemagglutination techniques, some of which became progeria famous as the Pregnosticon .
The shame of funk today! Honestly, I miss when funk spoke of political and social situation of the country, when ...
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